The purpose of the study was to explore the mechanism underlying the enhanced subthalamic nucleus (STN) neural activity during exhausting exercise from the perspective of monoamine neurotransmitters and changes of their corresponding receptors.Rats were randomly divided into microdialysis and immunohistochemistry study groups.For microdialysis study, extracellular fluid of the STN was continuously collected with a blue banana gorra microdialysis probe before, during and 90 min after one bout of exhausting exercise.
Dopamine (DA) and 5-hydroxytryptamine (5-HT) levels were subsequently detected with high-performance liquid chromatography (HPLC).For immunohistochemistry study, the expression of DRD 2 and HT 2C receptors in the STN, before, immediately after and 90 min after exhaustion was detected through immunohistochemistry technique.Microdialysis study results showed that the extracellular DA and 5-HT neurotransmitters increased significantly throughout the procedure of exhausting exercise and the recovery period (P0.
05).Our results suggest that the increased extracellular DA and 5-HT in the STN might be one important factor leading to the enhanced STN neural activity and the development of fatigue during exhausting exercise.This study may essentially offer useful evidence for better pbr keg understanding of the mechanism of the central type of exercise-induced fatigue.